3D Reconstruction of Kidney Cortex:
3D reconstruction of kidney cortex following administration of Texas Red-Gentamicin. Note endocytic uptake by proximal tubules cells and marked concentration in distal tubule lumens.

2-Photon Microscopy of Glomerular Filtration in a Living Rat:
Hoechst-labeled Nuclei, 500 Kd Fluorescein Dextran in Blood, 3 Kd Rhodamine Dextran in Bowman's Capsule and Tubule Lumens

Glomerular Filtration:

Glomerular filtration, proximal tubule endocytosis and concentration of a small molecular weight fluorescent dextran.

3D Reconstruction of Glomerular Capillaries with Ongoing Filtration and Blood Flow.

3D Reconstruction of Glomerular Capillaries with Ongoing Filtration and Blood Flow.

Renal Cortical Capillary Blood Flow

3D Reconstruction of a GFP-Producing Subcutaneous Tumor with Vascularization Outlined by a Large MW Red Fluorescent Dextran.

Multi-photon Microscopy of the Kidney of a Living Rat:

Endosomes and Lysosomes in the Proximal Tubule

3D Reconstruction of Hepatocellular Uptake of a Fluorescent Agent.

Image Segmentation:

3D Amira Segmentation of a Neuron